CRISPOR

DNA break repair outcome predictions

Guide: CGTCTTGTCACCATAGCCAA TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CTGCCCAGCCATGTATGTGGCGTCTTGTCACCATAGCCAATGGTCGTCAGTGTGATCTGGAGATACAAACAGGTTGCCCA`	(Wild-type sequence)
232	`ATGTATGTGGCGTCTTGTCA-------------------GTGTGATCTGGAGATACAAAC`	`19 bp deleted → frameshift`
224	`ATGTATGTGGCGTCTTGTC----------------GTCAGTGTGATCTGGAGATACAAAC`	`16 bp deleted → frameshift`
210	`ATGTATGTGGCGTC------------------------AGTGTGATCTGGAGATACAAAC`	`24 bp deleted → no frameshift`
182	`ATGTATGTGGCGTCTTGTCACCATAGCCA----------GTGTGATCTGGAGATACAAAC`	`10 bp deleted → frameshift`
175	`ATGTATGTGGCGTC---------------------GTCAGTGTGATCTGGAGATACAAAC`	`21 bp deleted → no frameshift`
158	`ATGTATGTGG-----------------------TCGTCAGTGTGATCTGGAGATACAAAC`	`23 bp deleted → frameshift`
149	`ATGTATGTGGCGTCTTGTCACCATAG--------------TGTGATCTGGAGATACAAAC`	`14 bp deleted → frameshift`
141	`ATGTATGTGGCGTCTTG---------------GTCGTCAGTGTGATCTGGAGATACAAAC`	`15 bp deleted → no frameshift`
134	`ATGTATGTGGCGTCTTGTCACCA----------------GTGTGATCTGGAGATACAAAC`	`16 bp deleted → frameshift`
114	`ATGTATGTGGCGTCTTGT-------------------------GATCTGGAGATACAAAC`	`25 bp deleted → frameshift`
104	`ATGTATGTG-----------------------------------ATCTGGAGATACAAAC`	`35 bp deleted → frameshift`
98	`ATGTATGTGGCGTCTTGTCACCATAGCCAA----------------------------AC`	`28 bp deleted → frameshift`
96	`ATGTATGTG---------------------------------TGATCTGGAGATACAAAC`	`33 bp deleted → no frameshift`
95	`ATGTATGTGGCGTCTTGT-----------------------GTGATCTGGAGATACAAAC`	`23 bp deleted → frameshift`
86	`ATGTATG-------------------------GTCGTCAGTGTGATCTGGAGATACAAAC`	`25 bp deleted → frameshift`
81	`ATGTATGTGGCGTCTTGTCACCATAG--------------------------ATACAAAC`	`26 bp deleted → frameshift`
81	`ATGTATGT--------------------------CGTCAGTGTGATCTGGAGATACAAAC`	`26 bp deleted → frameshift`
77	`ATGTATGTGGCGTCTTG---------------------------ATCTGGAGATACAAAC`	`27 bp deleted → no frameshift`
76	`ATGTATGTGGCGTCT---------------------------------GGAGATACAAAC`	`33 bp deleted → no frameshift`
74	`ATGTATGTGGCGTCTTGTC----------------------------TGGAGATACAAAC`	`28 bp deleted → frameshift`
74	`ATGTATGTGGCGT----------------------------GTGATCTGGAGATACAAAC`	`28 bp deleted → frameshift`
70	`ATGTATGT-----------------------------CAGTGTGATCTGGAGATACAAAC`	`29 bp deleted → frameshift`
70	`ATG-----------------------------GTCGTCAGTGTGATCTGGAGATACAAAC`	`29 bp deleted → frameshift`
67	`ATGTATGTGG----------------------------------------AGATACAAAC`	`40 bp deleted → frameshift`
66	`ATGTATGTGGCGTCTTGTCACCATA------------------------------CAAAC`	`30 bp deleted → no frameshift`
66	`ATGTATGTGGCGT------------------------------GATCTGGAGATACAAAC`	`30 bp deleted → no frameshift`
66	`ATGT------------------------------CGTCAGTGTGATCTGGAGATACAAAC`	`30 bp deleted → no frameshift`
66	`ATGTATGTGGCGTCTTGTCACCAT----------------------CTGGAGATACAAAC`	`22 bp deleted → frameshift`
60	`ATGTATGTGGCGTCTTG--------------------------------GAGATACAAAC`	`32 bp deleted → frameshift`
57	`ATGT---------------------------------CAGTGTGATCTGGAGATACAAAC`	`33 bp deleted → no frameshift`
56	`ATGT---------------------------------------GATCTGGAGATACAAAC`	`39 bp deleted → no frameshift`
54	`ATGTATGTGGCGTCTTGTCACCA----------------------------------AAC`	`34 bp deleted → frameshift`
52	`ATGTATGTGGCGTCTTGTCAC-----------------------------------AAAC`	`35 bp deleted → frameshift`
47	`ATGTATGTGGCGTCTTGTCA-------------------------------------AAC`	`37 bp deleted → frameshift`
47	`ATGTATG-------------------------------------ATCTGGAGATACAAAC`	`37 bp deleted → frameshift`
47	`ATGT-------------------------------------GTGATCTGGAGATACAAAC`	`37 bp deleted → frameshift`
47	`ATGTATGTGGCGTCTTGTCACCATAGCCAA-----------------------------C`	`29 bp deleted → frameshift`
42	`ATGTATGTGGCGTCTTGTCAC---------------------------------------`	`39 bp deleted → no frameshift`
38	`ATG-----------------------------------------ATCTGGAGATACAAAC`	`41 bp deleted → frameshift`
36	`ATGTATG------------------------------------------GAGATACAAAC`	`42 bp deleted → no frameshift`
30	`ATG----------------------------------------------GAGATACAAAC`	`46 bp deleted → frameshift`
27	`ATGTAT----------------------------------------CTGGAGATACAAAC`	`40 bp deleted → frameshift`
22	`AT--------------------------------------------CTGGAGATACAAAC`	`44 bp deleted → frameshift`
18	`ATGTAT------------------------------------------------ACAAAC`	`48 bp deleted → no frameshift`
16	`ATGTA--------------------------------------------------CAAAC`	`50 bp deleted → frameshift`
14	`AT----------------------------------------------------ACAAAC`	`52 bp deleted → frameshift`

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